Equipment for 1D and 2D agar and PAGE electrophoresis and electroblotting
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad, Biometra, VWR
Number of pieces:
Year of production: 2005 - 2009
Usage:
Agarose electrophoresis - 1D electrophoresis (RNA, DNA) with the possibility of the analysis of a large number of samples, 2D electrophoresis (using intercalating agents), temperature (TGGE) and denaturing gradient gel electrophoresis (DGGE).
Polyacrylamide electrophoresis - 1D separation of nucleic acids and proteins, the possibility of preparing gradient gels, native electrophoresis, 2D electrophoresis (native / SDS-PAGE, IEF / SDS-PAGE denaturing / SDS-PAGE), isoelectric focusing.
Elektroblotting – tank and semi-dry blotting.
Specifications
The laboratory has a large amount of electrophoretic resources to meet the needs of individual applications. For electrophoretic separation it is possible to use purchased (i.e. Precast) gels and gels prepared in the laboratory including gradient acrylamide gels for SDS-PAGE and native (BN-PAGE). Agarose electrophoresis enables the simultaneous analysis of more than 150 samples (if necessary, one analysis per about half an hour) for the detection of nucleic acids we mainly used SYBR Green (lower toxicological risk), but other detection methods can also be applied. Nucleic acids can be analyzed using denaturing and temperature gradient methods (a more accurate analysis of mutations and supercoiling in circular molecules) and also the RLB method (reverse-line blot). The laboratory is also equipped for the blotting of nucleic acids and their further analysis on membranes. For polyacrylamide electrophoresis (PAGE) single-concentration and gradient gels can be used; we are able to prepare all types of gels thanks to supplementary equipment. Furthermore, isoelectric focusing, denaturing or native electrophoresis in combination with SDS-PAGE as a 2D protein electrophoresis can be used.
Manual:
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/10007296.PDF
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_9002.pdf
Various
Extern: No
Transfer: No
