Preparation and purification of recombinant proteins

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Specifications

DNA amplification of the desired protein, cloning into plasmids (according to the tags, necessary for the purification of the resulting protein and the requirements for their presence), expression and subsequent purification of a recombinant protein.

Sample:

Conditions

According to the type and the number of proteins, used tags.

Identification of differentially expressed genes in various conditions

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Specifications

Cloning of cDNA libraries from organisms / samples formed under different conditions and the subsequent identification of differentially expressed genes using PCR.

Sample:

Conditions

According to the type and number of samples

Detection of the glycemic profile of an organism / sample

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Specifications

Identification of glycans present in a sample using mass spectrometry and associated methods.

Sample:

Conditions

According to the number of samples, type of samples, their volume.

Determination of B. burgdorferi strains in a sample

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Specifications

Determination of Borrelia burgdorferi strains which are present in a sample of molecular biological methods with greater accuracy as compared with single PCR and real-time PCR identification.

Sample:

Conditions

According to the number of samples

Identification of pathogens in ticks

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Specifications

Identification of various pathogens in ticks (or in other samples) using molecular biological methods.

Sample:

Conditions

According to the number of samples, the type and number of the identified pathogens.

Basic laboratory equipment

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout

Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Instrument

Manufacturer: Labnet, Bioer, Eppendorf, Gilson, Orange Scientific, Hettich, VWR
Number of pieces:
Year of production: 2000-2010
Usage:

Basic equipment for the preparation and processing of samples.

Specifications

The basic equipment consists of small appliances and other equipment necessary for work in the laboratory such as automatic pipettes, pipettors, centrifuges, vortexes (including attachments), shakers, pH meters, scales and analytical balances, sonicators, ultrasonic baths, dry blocks, shaking dry blocks, hybridization ovens and others.

Manual:

Various

Extern: No

Transfer: No

Terms of lease:

PCR boxes

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout

Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Instrument

Manufacturer:
Number of pieces: 2
Year of production: 2008, 2009
Usage:

Preparation of PCR reactions in clean conditions, thereby preventing contamination and false-positive results.

Specifications

Equipped with UV sterilization, HEPA filtration of intake air.

Manual:

Various

Extern: No

Transfer: No

Terms of lease:

Incubators

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout

Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Instrument

Manufacturer: Nüve
Number of pieces: 4
Year of production: 1980-2010
Usage:

Cultivation of cell cultures / tissues and carrying out the reactions under stable temperature conditions. The possibility of using CO2 sealed plastic boxes for cultivating cell cultures requiring more demanding conditions.

Specifications

The laboratory has 3 incubators without refrigeration options (manual and electronic control) and 1-cooled incubator (electronic control units).

Manual:

http://www.nuve.com.tr/images/urun/pdf/es_120_-_new.pdf

Various

Extern: No

Transfer: No

Terms of lease:

Homogeniser

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout

Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Instrument

Manufacturer: Qiagen
Number of pieces: 1
Year of production: 2009
Usage:

Enables the standardised homogenisation of biological samples.

Specifications

The homogenizer operates with a frequency of 30 shakes per second, homogenization takes place using autoclavable metal balls, with the possibility of the simultaneous homogenisation of 48 samples. In case of need it is also possible to homogenize under reduced temperatures. Time of homogenization is up to 5 minutes.

Manual:

http://www.qiagen.com/literature/render.aspx?id=200069

Various

Extern: No

Transfer: No

Terms of lease:

Biohazard flow-box

Place

University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice

The guarantor

Name and surname: František Adamec, technological scout

Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

Instrument

Manufacturer: Telstar
Number of pieces: 2
Year of production: 2007, 2010
Usage:

Allows you to work in a clean environment, prevents the contamination of samples and also protects workers. Suitable for working with infectious and highly infectious material, also for the processing of any contamination-sensitive samples.

Specifications

Flow-box is equipped with HEPA filtration intake and exhaust air (HEPA 99.999% efficiency), a motorized window with a seal, UV sterilization, a gas burner, a backup battery for closing the window in case of a power failure, electronic control and online monitoring of the functionality of the HEPA filters and the other parts of the box.

Manual:

http://www.telstar-lifesciences.com/en/technologies/laminar+air+flow+systems/biosafety+cabinets/bioultra.htm

Various

Extern: No

Transfer: No

Terms of lease: